Thioredoxin Reductase Protein (TrxR) (AA 1-321)
This Recombinant Thioredoxin Reductase protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for Thioredoxin Reductase Protein (TrxR) (AA 1-321) (ABIN666984)
抗原
See all Thioredoxin Reductase (TrxR) 蛋白
Thioredoxin Reductase (TrxR)
蛋白类型
Recombinant
生物活性
Active
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
纯度
> 90 %
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产品特性
- AA 1-321
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序列
- MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K
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产品特性
- TRXB, 1-321aa, E.coli, E.coli
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纯化方法
- > 90% by SDS-PAGE
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Biological Activity Comment
- Specific activity is > 10unit/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.
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Thioredoxin Reductase (TrxR) (AA 1-321) (Active) protein
TrxR 宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Thioredoxin Reductase (TrxR)
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别名
- trxB
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背景
- TRXB (Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E. coli TRXB protein was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 34.6 kDa (321aa) confirmed by MALDI-TOF
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NCBI登录号
- YP_003044110
抗原
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